Secondary chilled syringe for protein handling Trajan ProDx trap columns optimized for low temperatureįorward compatible capacity for future models Trajan ProDx protease columns optimized for low temperature Independent protease column temperature control Optimized valve fluidics for inline digestion and automatic backflushing Vial capacity for 100 time point experiments Optimized fittings and precut tubing ensure maximum reliability and instrument up-time.Īutomatic syringe selection for extended dynamic range Trajan’s LEAP HDX platform incorporates an advanced cooling chamber for the trapping and chromatography phases which includes an enclosed valve and column chiller as well as solvent pre-conditioning.ĭigestion temperature is controlled independently with the protease column chamber, to allow improved digestion efficiency.Ī special 3 valve configuration enables zero-downtime backflushing for retention time stability, extended column life and superior carryover protection. Unique fluidics automation for the best performance
Cap of the undisturbed mind software#
The Trajan LEAP HDX platform works natively with HDExaminer from Sierra Analytics, a complete software solution for HDX analysis, independent of MS data type. HDExaminer: a complete analytical tool for HDX experiments Our Chronos for HDX software offers a streamlined interface for HDX users and automatically overlaps time points for maximum MS efficiency.Ĭombines ease of use with advanced options, allowing promotion of user-defined parameters to the sample list, giving the operator control over all aspects of the method without complication. Sustained 0☌ environment reduces back exchange, improves sample stability and overall reproducibility. The most efficient system control, with unique temperature set points at all stages of the experiment to ensure reliable data. With HDExaminer, swiftly and easily analyze data from any MS instrument to get a complete picture of the overall deuteration behavior of your protein. Move more quickly, make better decisions.
Powerful information on protein structure in days – not weeks.
Cap of the undisturbed mind manual#
Our total HDX platform covers the complete process: sample input > the many meticulous steps of preparation > optimizing mass spec utilization > clear answers with minimal manual data curation. Brochure - Automated Hydrogen-Deuterium Exchange (Japanese).Brochure - Automated Hydrogen-Deuterium Exchange.Trajan’s HDX system is compatible with bottom-up, top- and middle-down protein analysis studies. HDX is an indispensable complementary tool for protein biophysics. It can be used to replicate critical native state conditions, for the most useful results. In addition to being faster than other protein techniques like x-ray crystallography and NMR, HDX data can be obtained from samples that are challenging to crystalize and from samples in short supply.
HDX uses variations in deuterium exchange rates between more and less accessible/structured regions throughout a protein. HDX is a well-established analytical technique for protein-ligand, protein-protein, protein-conjugate interactions and protein stability studies. In-line digestion – fully leverages and identifies ideal digestion conditions.Sustained 0☌ environment – precision at temperatures from 0☌ to biological conditions.Maintained pH control – optimizes quench conditions for maximum sequence coverage.Reproducible accuracy in timing of liquid transfers – overlapped to ensure efficiency.With robotic precision, Trajan’s HDX platform exerts exacting control of all key factors: Successful HDX experiments are all about control HDX, especially automated HDX, has uses in development, manufacturing and post-production, providing insight into your protein’s tertiary structure, in its native state(s). Features and benefitsĪutomated HDX provides structural confidence in native states Trajan’s LEAP HDX system is the recognized leader, preferred around the globe by scientists requiring confidence in reproducible results. Since 2002 Trajan has been automating HDX, providing continuous enhancements for rugged HDX sample control and scheduling.
Contact a workflow specialist for your specific needs Flexibility by design
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